Identification of homologous protein models via 3D comparisons using predicted structures.
STAR Protoc
; 5(1): 102814, 2024 Mar 15.
Article
en En
| MEDLINE
| ID: mdl-38183654
ABSTRACT
Recent advances in protein structure prediction enable 3D homology alignment and domain annotation using tertiary structures. Here, we present a protocol to identify homologous structures and annotate protein domains through in silico comparisons using the AlphaFold database. We describe steps for downloading and installing PyMOL software, preparing the query structure, and conducting a 3D homology search. The example provided highlights the application of this protocol in reevaluating an mpox viral protein annotation. For complete details on the use and execution of this protocol, please refer to Pan et al. (2023).1.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Programas Informáticos
/
Proteínas
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
/
Risk_factors_studies
Idioma:
En
Revista:
STAR Protoc
Año:
2024
Tipo del documento:
Article
País de afiliación:
China