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A COX2-targeting cancer-specific fluorescent probe for hydrogen sulfide detection in living cells, Caenorhabditis elegans, and zebrafish.
Yuan, Fengying; Zhao, Qiao; Zeng, Yanyan; Liao, Xu Fang; Li, Jiali; Liu, Bo; Kou, Jun Feng; Zhong, Xiaolin; Wu, Xiang Hua; Zhang, Jun Feng; Ren, Wen Xiu.
Afiliación
  • Yuan F; Department of Radiology, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China. xrenwenxiux@swmu.edu.cn.
  • Zhao Q; Functional and Molecular Imaging Key Laboratory of Sichuan Province, Chengdu 610000, China.
  • Zeng Y; College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650500, China. chxhwu@sina.com.
  • Liao XF; Department of Radiology, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China. xrenwenxiux@swmu.edu.cn.
  • Li J; College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650500, China. chxhwu@sina.com.
  • Liu B; Department of Radiology, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China. xrenwenxiux@swmu.edu.cn.
  • Kou JF; College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650500, China. chxhwu@sina.com.
  • Zhong X; College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650500, China. chxhwu@sina.com.
  • Wu XH; Department of Gastroenterology, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
  • Zhang JF; College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650500, China. chxhwu@sina.com.
  • Ren WX; College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650500, China. chxhwu@sina.com.
Analyst ; 149(5): 1489-1495, 2024 Feb 26.
Article en En | MEDLINE | ID: mdl-38314794
ABSTRACT
A novel cyclooxygenase-2 (COX-2) targeted H2S-activated cancer-specific fluorescent probe, namely, COX2-H2S, was designed and synthesized, with naphthalimide as the fluorophore and indomethacin as the targeting group. This H2S-sensing probe was developed to differentiate tumor cells from normal cells and was tested in living cells, Caenorhabditis elegans (C. elegans), and zebrafish. The probe could successfully be used for imaging endogenous and exogenous H2S in living cells, demonstrating high sensitivity and specificity and strong anti-interference. COX2-H2S had the ability to not only discern cancer cells from normal cells but also specifically recognize 9L/lacZ cells from other glioblastoma cells (U87-MG and LN229). It could also be successfully applied for the fluorescent live imaging of H2S in both C. elegans and zebrafish.
Asunto(s)

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Sulfuro de Hidrógeno / Neoplasias Tipo de estudio: Diagnostic_studies Límite: Animals / Humans Idioma: En Revista: Analyst Año: 2024 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Sulfuro de Hidrógeno / Neoplasias Tipo de estudio: Diagnostic_studies Límite: Animals / Humans Idioma: En Revista: Analyst Año: 2024 Tipo del documento: Article País de afiliación: China