The prevalence of controls in phyllosphere microbiome research: a methodological review.
New Phytol
; 242(1): 23-29, 2024 Apr.
Article
en En
| MEDLINE
| ID: mdl-38339825
ABSTRACT
DNA contamination can critically confound microbiome studies. Here, we take a systematic approach to review the current literature and investigate the prevalence of contamination controls in phyllosphere microbiome research over the past decade. By utilising systematic review principles for this review, we were able to conduct a thorough investigation, screening 450 articles from three databases for eligibility and extracting data in a controlled and methodical manner. Worryingly, we observed a surprisingly low usage of both positive and negative contamination controls in phyllosphere research. As a result, we propose a set of minimum standards to combat the effects of contamination in future phyllosphere research.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Microbiota
Tipo de estudio:
Prevalence_studies
/
Risk_factors_studies
Idioma:
En
Revista:
New Phytol
Asunto de la revista:
BOTANICA
Año:
2024
Tipo del documento:
Article
País de afiliación:
Australia