A gene-encoded aldehyde tag repurposed from RiPP cyclophane-forming pathway.

Ma, Suze; Chen, Heng; Liu, Shuxun; Huang, Xuedong; Mo, Tianlu; Liu, Wan-Qiu; Zhang, Wei; Ding, Wei; Zhang, Qi

Ma, Suze; Chen, Heng; Liu, Shuxun; Huang, Xuedong; Mo, Tianlu; Liu, Wan-Qiu; Zhang, Wei; Ding, Wei; Zhang, Qi.

Afiliación

Ma S; Department of Chemistry, Fudan University, Shanghai 200433, China.
Chen H; Department of Chemistry, Fudan University, Shanghai 200433, China; State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Liu S; Department of Chemistry, Fudan University, Shanghai 200433, China.
Huang X; Department of Chemistry, Fudan University, Shanghai 200433, China.
Mo T; School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
Liu WQ; School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Zhang W; Key Laboratory of Extreme Environmental Microbial Resources and Engineering of Gansu Province, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, China.
Ding W; State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Zhang Q; Department of Chemistry, Fudan University, Shanghai 200433, China. Electronic address: qizhang@sioc.ac.cn.

Bioorg Med Chem Lett ; 101: 129653, 2024 Mar 15.

Article en En | MEDLINE | ID: mdl-38360420

ABSTRACT

ABSTRACT

Gene-encoded aldehyde tag technology has been widely utilized in protein bioorthogonal chemistry and biotechnological application. Herein, we report utilization of the promiscuous rSAM cyclophane synthase SjiB involved in triceptide biosynthesis as a dedicated and highly efficient formylglycine synthase. The new aldehyde tag sequence in this system, YQSSI, is biosynthetically orthogonal to the known aldehyde tag (C/S)x(P/A)xR. The potential use of SjiB/YQSSI aldehyde tag system was further validated in fluorescent labelling of model proteins.

Asunto(s)

Aldehídos; Ciclofanos; Proteínas

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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Aldehídos / Ciclofanos Idioma: En Revista: Bioorg Med Chem Lett Asunto de la revista: BIOQUIMICA / QUIMICA Año: 2024 Tipo del documento: Article País de afiliación: China

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