Multi-tissue transcriptome-wide association studies identified 235 genes for intrinsic subtypes of breast cancer.
J Natl Cancer Inst
; 116(7): 1105-1115, 2024 Jul 01.
Article
en En
| MEDLINE
| ID: mdl-38400758
ABSTRACT
BACKGROUND:
Although genome-wide association studies (GWAS) of breast cancer (BC) identified common variants which differ between intrinsic subtypes, genes through which these variants act to impact BC risk have not been fully established. Transcriptome-wide association studies (TWAS) have identified genes associated with overall BC risk, but subtype-specific differences are largely unknown.METHODS:
We performed two multi-tissue TWAS for each BC intrinsic subtype, including an expression-based approach that collated TWAS signals from expression quantitative trait loci (eQTLs) across multiple tissues and a novel splicing-based approach that collated signals from splicing QTLs (sQTLs) across intron clusters and subsequently across tissues. We used summary statistics for five intrinsic subtypes including Luminal A-like, Luminal B-like, Luminal B/HER2-negative-like, HER2-enriched-like, and triple-negative BC, generated from 106â278 BC cases and 91â477 controls in the Breast Cancer Association Consortium.RESULTS:
Overall, we identified 235 genes in 88 loci that were associated with at least one of the five intrinsic subtypes. Most genes were subtype-specific, and many have not been reported in previous TWAS. We discovered common variants that modulate expression of CHEK2 confer increased risk to Luminal A-like BC, in contrast to the viewpoint that CHEK2 primarily harbors rare, penetrant mutations. Additionally, our splicing-based TWAS provided population-level support for MDM4 splice variants that increased the risk of triple-negative BC.CONCLUSION:
Our comprehensive, multi-tissue TWAS corroborated previous GWAS loci for overall BC risk and intrinsic subtypes, while underscoring how common variation that impacts expression and splicing of genes in multiple tissue types can be used to further elucidate the etiology of BC.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Neoplasias de la Mama
/
Sitios de Carácter Cuantitativo
/
Estudio de Asociación del Genoma Completo
/
Transcriptoma
Límite:
Female
/
Humans
Idioma:
En
Revista:
J Natl Cancer Inst
/
J. natl. cancer inst
/
Journal of the national cancer institute
Año:
2024
Tipo del documento:
Article
País de afiliación:
Estados Unidos