Iridium(III)-based minor groove binding complexes as DNA photocleavage agents.
Dalton Trans
; 53(17): 7282-7291, 2024 Apr 30.
Article
en En
| MEDLINE
| ID: mdl-38466178
ABSTRACT
Transition metal complexes containing the qtpy ligand (2'4,4''4',4'''-quaterpyridyl) are known to be DNA intercalators or minor groove binders. In this study, new tricationic iridium(III) complexes of qtpy are reported. Both [Ir(bpy)2(qtpy)]3+1 and [Ir(phen)2(qtpy)]3+2 display good water solubility as chloride salts. The complexes possess high-energy excited states, which are quenched in the presence of duplex DNA and even by the mononucleotides guanosine monophosphate and adenosine monophosphate. Further studies reveal that although the complexes bind to quadruplex DNA, they display a preference for duplex structures, which are bound with an order of magnitude higher affinities than their isostructural dicationic RuII-analogues. Detailed molecular dynamics simulations confirm that the complexes are groove binders through the insertion of, predominantly, the qtpy ligand into the minor groove. Photoirradiation of 1 in the presence of plasmid DNA confirms that this class of complexes can function as synthetic photonucleases by cleaving DNA.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
ADN
/
Complejos de Coordinación
/
Iridio
Idioma:
En
Revista:
Dalton Trans
Asunto de la revista:
QUIMICA
Año:
2024
Tipo del documento:
Article
País de afiliación:
Reino Unido