ALDH Activity Assay: A Method for Cancer Stem Cell (CSC) Identification and Isolation.
Methods Mol Biol
; 2777: 83-89, 2024.
Article
en En
| MEDLINE
| ID: mdl-38478337
ABSTRACT
Cancer stem cells (CSCs) are a small tumor cell subpopulation, driving cancer initiation, progression, multidrug resistance, and metastasis. Several methods are used to detect and isolate CSCs by flow cytometry. Among these, measurement of aldehyde dehydrogenase (ALDH) activity within the cell is an assay widely used to identify and isolate CSCs from different types of solid tumors. The aldehyde dehydrogenase (ALDH) is a polymorphic enzyme responsible for the oxidation of aldehydes to carboxylic acids, overexpressed both in normal and cancer stem cells. In this chapter, it is described how CSCs are detected and isolated by using ALDH activity assay.
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Bases de datos:
MEDLINE
Asunto principal:
Células Madre Neoplásicas
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Neoplasias
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2024
Tipo del documento:
Article
País de afiliación:
Italia