Transparency-enhancing technology allows the three-dimensional assessment of esophageal carcinoma obtained by endoscopic submucosal dissection.
Esophagus
; 21(3): 405-409, 2024 Jul.
Article
en En
| MEDLINE
| ID: mdl-38498095
ABSTRACT
BACKGROUND:
Although much progress has been made in diagnosis of carcinomas, no established methods have been confirmed to elucidate their morphological features.METHODS:
Three-dimensional structure of esophageal carcinomas was assessed using transparency-enhancing technology. Endoscopically resected esophageal squamous cell carcinoma was fluorescently stained, optically cleared using a transparency-enhancing reagent called LUCID, and visualized using laser scanning microscopy. The resulting microscope images were converted to virtual HE images for observation using ImageJ software.RESULTS:
Microscopic observation and image editing enabled three-dimensional image reconstruction and conversion to virtual HE images. The structure of abnormal blood vessels in esophageal carcinoma recognized by endoscopy could be observed in the 3 dimensions. Squamous cell carcinoma and normal squamous epithelium could be distinguished in the virtual HE images.CONCLUSIONS:
The results suggested that transparency-enhancing technology and virtual HE images may be feasible for clinical application and represent a novel histopathological method for evaluating endoscopically resected specimens.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Neoplasias Esofágicas
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Microscopía Confocal
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Imagenología Tridimensional
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Resección Endoscópica de la Mucosa
Límite:
Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Esophagus
Año:
2024
Tipo del documento:
Article
País de afiliación:
Japón