Circular RNA hsa_circ_0005519 contributes to acute kidney injury via sponging microRNA-98-5p.
BMC Nephrol
; 25(1): 107, 2024 Mar 19.
Article
en En
| MEDLINE
| ID: mdl-38504194
ABSTRACT
BACKGROUND:
This study intends to explore the role and molecular mechanism of hsa_circ_0005519 in acute kidney injury (AKI).METHODS:
We conducted reverse transcription-qPCR for human serum to determine levels of hsa_circ_0005519 in AKI patients and healthy controls. Hsa_circ_0005519 was inhibited for expression in HK-2 cells using specific siRNAs. A number of techniques, MTT and ELISA assays, were used to analyze the potential role of hsa_circ_0005519 in cell viability, oxidative stress, and inflammation of LPS-induced HK-2 cells.RESULTS:
The serum of patients with AKI exhibited a significant increase in hsa_circ_0005519 expression, compared with healthy controls. Hsa_circ_0005519 was knockdown by siRNA, and its knockdown led to cell viability increase in LPS-induced HK-2 cells. Inhibition of hsa_circ_0005519 can reverse the TNF-α, IL-6 and IL-1ß increase in LPS-induced HK-2 cells. Inhibiting hsa_circ_0005519 led to downregulation of MPO and MDA levels. MiR-98-5p was a downstream miRNA for hsa_circ_0005519. MiR-98-5p can offset the effects of hsa_circ_0005519 on LPS-induced HK-2 cells. IFG1R was a target gene for miR-98-5p.CONCLUSIONS:
These findings indicate that the highly expressed hsa_circ_0005519 plays a promoting role in AKI.Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
MicroARNs
/
Lesión Renal Aguda
Límite:
Humans
Idioma:
En
Revista:
BMC Nephrol
/
BMC nephrology
Asunto de la revista:
NEFROLOGIA
Año:
2024
Tipo del documento:
Article
País de afiliación:
China