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Exploring the antibiofilm effects on Escherichia coli biofilm associated with colon cancer and anticancer activities on HCT116 cell line of bee products.
Akkoyunlu, Aysegül; Dülger, Görkem.
Afiliación
  • Akkoyunlu A; Department of Biology, Graduate Education Institute, Duzce University, Konuralp/Düzce, Turkey.
  • Dülger G; Department of Medical Biology, Faculty of Medicine, Duzce University, Konuralp/Düzce, Turkey.
Biofouling ; 40(3-4): 235-244, 2024.
Article en En | MEDLINE | ID: mdl-38584359
ABSTRACT
The association between dysbiotic microbiota biofilm and colon cancer has recently begun to attract attention. In the study, the apitherapeutic effects of bee products (honey, bee venom, royal jelly, pollen, perga and propolis) obtained from the endemic Yigilca ecotype of Apis mellifera anatoliaca were investigated. Antibiofilm activity were performed by microplate assay using crystal violet staining to measure adherent biofilm biomass of Escherichia coli capable of forming biofilms. Bee venom showed the highest inhibition effect (73.98%) at 50% concentration. Honey, perga and royal jelly reduced biofilm formation by >50% at all concentrations. The antiproliferation effect on the HCT116 colon cancer cell line was investigated with the water­soluble tetrazolium salt­1 assay. After 48 h of honey application at 50% concentration, cell proliferation decreased by 86.51%. The high cytotoxic effects of royal jelly and bee venom are also remarkable. Additionally, apoptotic pathway analysis was performed by ELISA using caspase 3, 8 and 9 enzyme-linked immunosorbent assay kits. All bee products induced a higher expression of caspase 9 compared with caspase 8. Natural products that upregulate caspase proteins are promising therapeutic targets for proliferative diseases.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Própolis / Venenos de Abeja / Neoplasias del Colon / Biopelículas / Escherichia coli / Ácidos Grasos / Antineoplásicos Límite: Animals / Humans Idioma: En Revista: Biofouling Asunto de la revista: BIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Turquía

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Própolis / Venenos de Abeja / Neoplasias del Colon / Biopelículas / Escherichia coli / Ácidos Grasos / Antineoplásicos Límite: Animals / Humans Idioma: En Revista: Biofouling Asunto de la revista: BIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Turquía