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Simultaneous quantitative respirometry and fluorometric assays in dissected hippocampal tissue from mice.
Logan, Sreemathi; Ranjit, Rojina; Rose, Hadyn; Bredegaard, Anne; Díaz-García, Carlos Manlio.
Afiliación
  • Logan S; Department of Biochemistry and Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA; Center for Geroscience and Healthy Brain Aging, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA; Stephenson Cancer Center, University of Oklahoma Health Sciences Ce
  • Ranjit R; Department of Biochemistry and Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA; Center for Geroscience and Healthy Brain Aging, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
  • Rose H; Department of Biochemistry and Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA; Center for Geroscience and Healthy Brain Aging, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
  • Bredegaard A; Department of Biochemistry and Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA; Center for Geroscience and Healthy Brain Aging, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
  • Díaz-García CM; Department of Biochemistry and Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA; Center for Geroscience and Healthy Brain Aging, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA; Harold Hamm Diabetes Center, University of Oklahoma Health Sciences
STAR Protoc ; 5(2): 102988, 2024 Jun 21.
Article en En | MEDLINE | ID: mdl-38635395
ABSTRACT
Respirometry is a technique for studying mitochondrial function that has proven compatibility with ≥0.5 mg of brain tissue. Here, we present a protocol for assessing oxygen consumption and H2O2 production rates in hippocampal tissue using the Oroboros O2k system. We describe steps for brain harvesting, tissue preparation, hippocampal microdissection, and respirometry assays. This approach has been valuable to study the metabolism of dentate granule cells of the hippocampus and could be applicable to other brain subregions. For complete details on the use and execution of this protocol, please refer to Rose et al.1.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Consumo de Oxígeno / Fluorometría / Hipocampo / Mitocondrias Límite: Animals Idioma: En Revista: STAR Protoc Año: 2024 Tipo del documento: Article
Texto completo
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Consumo de Oxígeno / Fluorometría / Hipocampo / Mitocondrias Límite: Animals Idioma: En Revista: STAR Protoc Año: 2024 Tipo del documento: Article