[Age-dependent expression of HSP90 in the hippocampus of APP/PS1 mice].
Sheng Li Xue Bao
; 76(2): 257-265, 2024 Apr 25.
Article
en Zh
| MEDLINE
| ID: mdl-38658375
ABSTRACT
The present study aims to observe the change in expression of heat shock protein 90 (HSP90) along with amyloid-ß (Aß) and phosphorylated Tau (p-Tau) protein levels in the hippocampus tissue of Alzheimer's disease (AD) transgenic animal model with age. APP/PS1 transgenic mice at age of 6-, 9- and 12-month and C57BL/6J mice of the same age were used. The cognitive abilities of these animals were evaluated using a Morris water maze. Western blot or immunohistochemistry was used to detect the expressions of HSP90 and Aß1-42, as well as the phosphorylation levels of Tau protein in the hippocampus. The hsp90 mRNA levels and the morphology and number of cells in the hippocampus were detected with real-time quantitative polymerase chain reaction (qRT-PCR) and Nissl staining, respectively. The results showed that compared with C57BL/6J mice of the same age, HSP90 and hsp90 mRNA expression were decreased (P < 0.05 or P < 0.01), while Aß1-42 and p-Tau protein levels were increased (P < 0.05 or P < 0.01) in the hippocampal tissue of APP/PS1 transgenic mice. Meanwhile, the decrease in HSP90 and hsp90 mRNA expression (P < 0.05 or P < 0.01), the increase in Aß1-42 and p-Tau levels (P < 0.01 or P < 0.05) in hippocampal tissue and the reduction in behavioral ability showed a progressive development with the advancing of age in the APP/PS1 transgenic mice. In conclusion, in the hippocampal tissue of APP/PS1 mice, the decrease in HSP90 expression and the increase in Aß1-42 and p-Tau levels together with the decline of their cognitive ability are age-dependent.
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Bases de datos:
MEDLINE
Asunto principal:
Ratones Transgénicos
/
Péptidos beta-Amiloides
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Precursor de Proteína beta-Amiloide
/
Proteínas tau
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Proteínas HSP90 de Choque Térmico
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Enfermedad de Alzheimer
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Hipocampo
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Ratones Endogámicos C57BL
Límite:
Animals
Idioma:
Zh
Revista:
Sheng Li Xue Bao
Año:
2024
Tipo del documento:
Article
País de afiliación:
China