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Cell-engineered virus-mimetic nanovesicles for vaccination against enveloped viruses.
Han, Chungmin; Kim, Suyeon; Seo, Youngjoo; Lim, Minyeob; Kwon, Yongmin; Yi, Johan; Oh, Seung-Ik; Kang, Minsu; Jeon, Seong Gyu; Park, Jaesung.
Afiliación
  • Han C; School of Interdisciplinary Bioscience and Bioengineering (I-Bio), Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea.
  • Kim S; School of Interdisciplinary Bioscience and Bioengineering (I-Bio), Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea.
  • Seo Y; School of Interdisciplinary Bioscience and Bioengineering (I-Bio), Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea.
  • Lim M; School of Interdisciplinary Bioscience and Bioengineering (I-Bio), Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea.
  • Kwon Y; Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea.
  • Yi J; Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea.
  • Oh SI; School of Interdisciplinary Bioscience and Bioengineering (I-Bio), Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea.
  • Kang M; School of Interdisciplinary Bioscience and Bioengineering (I-Bio), Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea.
  • Jeon SG; Exosome Plus, Suwon, Republic of Korea.
  • Park J; School of Interdisciplinary Bioscience and Bioengineering (I-Bio), Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea.
J Extracell Vesicles ; 13(4): e12438, 2024 Apr.
Article en En | MEDLINE | ID: mdl-38659363
ABSTRACT
Enveloped viruses pose a significant threat to human health, as evidenced by the recent COVID-19 pandemic. Although current vaccine strategies have proven effective in preventing viral infections, the development of innovative vaccine technologies is crucial to fortify our defences against future pandemics. In this study, we introduce a novel platform called cell-engineered virus-mimetic nanovesicles (VNVs) and demonstrate their potential as a vaccine for targeting enveloped viruses. VNVs are generated by extruding plasma membrane-derived blebs through nanoscale membrane filters. These VNVs closely resemble enveloped viruses and extracellular vesicles (EVs) in size and morphology, being densely packed with plasma membrane contents and devoid of materials from other membranous organelles. Due to these properties, VNVs express viral membrane antigens more extensively and homogeneously than EVs expressing the same antigen. In this study, we produced severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) VNVs expressing the SARS-CoV-2 Spike glycoprotein (S) on their surfaces and assessed their preclinical efficacy as a COVID-19 vaccine in experimental animals. The administration of VNVs successfully stimulated the production of S-specific antibodies both systemically and locally, and immune cells isolated from vaccinated mice displayed cytokine responses to S stimulation.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Vesículas Extracelulares / Vacunas contra la COVID-19 / SARS-CoV-2 / COVID-19 Límite: Animals / Female / Humans Idioma: En Revista: J Extracell Vesicles Año: 2024 Tipo del documento: Article

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Vesículas Extracelulares / Vacunas contra la COVID-19 / SARS-CoV-2 / COVID-19 Límite: Animals / Female / Humans Idioma: En Revista: J Extracell Vesicles Año: 2024 Tipo del documento: Article