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Enhanced osteogenic differentiation potential of Arnica montana and Bellis perennis in C3H10T1/2 multipotent mesenchymal stem cells.
Deepika, Bhat K; Apoorva, Nagendra H; Joel, Pinto R; B, Bipasha; Sudheer, Shenoy P.
Afiliación
  • Deepika BK; Stem Cells and Regenerative Medicine Centre, Yenepoya Research Centre, Yenepoya Deemed to Be University, University Road, Deralakatte, Mangalore, Karnataka, 575018, India.
  • Apoorva NH; Stem Cells and Regenerative Medicine Centre, Yenepoya Research Centre, Yenepoya Deemed to Be University, University Road, Deralakatte, Mangalore, Karnataka, 575018, India.
  • Joel PR; Stem Cells and Regenerative Medicine Centre, Yenepoya Research Centre, Yenepoya Deemed to Be University, University Road, Deralakatte, Mangalore, Karnataka, 575018, India.
  • B B; Stem Cells and Regenerative Medicine Centre, Yenepoya Research Centre, Yenepoya Deemed to Be University, University Road, Deralakatte, Mangalore, Karnataka, 575018, India.
  • Sudheer SP; Stem Cells and Regenerative Medicine Centre, Yenepoya Research Centre, Yenepoya Deemed to Be University, University Road, Deralakatte, Mangalore, Karnataka, 575018, India. shenoy@yenepoya.edu.in.
Mol Biol Rep ; 51(1): 596, 2024 Apr 29.
Article en En | MEDLINE | ID: mdl-38683461
ABSTRACT

BACKGROUND:

Arnica montana and Bellis perennis are two medicinal plants that are thought to accelerate bone repair in homoeopathic literature. Mesenchymal stem cells (MSCs) are multipotent stem cells with the ability to differentiate and regenerate bone or osteogenesis. Hence, we aimed to determine the role of Arnica montana and Bellis perennis on the osteogenic differentiation of the C3H10T1/2 stem cell line. METHODS AND

RESULTS:

The cell proliferation of Arnica montana and Bellis perennis was evaluated by MTT assay. Osteogenic differentiation of C3H10T1/2 was induced by the addition of ß-glycerophosphate, ascorbic acid and dexamethasone in the differentiation medium over 3 weeks. Cells were treated with Arnica montana and Bellis perennis individually as well as in combination. The osteogenic differentiation potential of Arnica montana and Bellis perennis to differentiate C3H10T1/2 into osteoblasts was measured by alkaline phosphatase activity, alizarin red staining and the expression of Osteocalcin using immunostaining and qRT-PCR. Arnica montana and Bellis perennis could enhance C3H10T1/2 cell proliferation at 1600 µg. Further, the compound showed the ability to augment osteogenesis as confirmed by increased expression of alkaline phosphatase and enhanced calcium accumulation as seen by the Alizarin Red staining and quantification. Enhanced osteogenesis was further supported by the increased expression of osteocalcin in the treated cells with individual and combined doses of Arnica montana and Bellis perennis. Therefore, the findings provide additional support for the positive impact of Arnica montana and Bellis perennis on bone formation.

CONCLUSIONS:

Our findings suggest that homoeopathic compounds Arnica montana and Bellis perennis can augment osteogenesis individually as well as in combination.
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Osteogénesis / Extractos Vegetales / Diferenciación Celular / Arnica / Proliferación Celular / Células Madre Mesenquimatosas Límite: Animals Idioma: En Revista: Mol Biol Rep Año: 2024 Tipo del documento: Article País de afiliación: India

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Osteogénesis / Extractos Vegetales / Diferenciación Celular / Arnica / Proliferación Celular / Células Madre Mesenquimatosas Límite: Animals Idioma: En Revista: Mol Biol Rep Año: 2024 Tipo del documento: Article País de afiliación: India