Comparison of peripheral blood automated hematopoietic progenitor cell count and flow cytometric CD34+ cell count.
J Clin Apher
; 39(3): e22114, 2024 Jun.
Article
en En
| MEDLINE
| ID: mdl-38708583
ABSTRACT
BACKGROUND:
Stem cell apheresis in the context of autologous stem cell transplantation requires an accurate cluster of differentiantion 34 (CD34+) count determined by flow cytometry as the current gold standard. Since flow cytometry is a personnel and time-intensive diagnostic tool, automated stem cell enumeration may provide a promising alternative. Hence, this study aimed to compare automated hematopoietic progenitor enumeration carried out on a Sysmex XN-20 module compared with conventional flow cytometric measurements.METHODS:
One hundred forty-three blood samples from 41 patients were included in this study. Correlation between the two methods was calculated over all samples, depending on leukocyte count and diagnosis.RESULTS:
Overall, we found a high degree of correlation (r = 0.884). Furthermore, correlation was not impaired by elevated leukocyte counts (>10 000/µL, r = 0.860 vs <10 000/µL, r = 0.849; >20 000/µL, r = 0.843 vs <20 000/µL, r = 0.875). However, correlation was significantly impaired in patients with multiple myeloma (multiple myeloma r = 0.840 vs nonmyeloma r = 0.934).SUMMARY:
Stem cell measurement carried out on the Sysmex XN-20 module provides a significant correlation with flow cytometry and might be implemented in clinical practice. In clinical decision-making, there was discrepancy of under 15% of cases. In multiple myeloma patients, XN-20 should be used with caution.Palabras clave
Texto completo:
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Bases de datos:
MEDLINE
Asunto principal:
Células Madre Hematopoyéticas
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Antígenos CD34
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Citometría de Flujo
Límite:
Adult
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Female
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Humans
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Male
Idioma:
En
Revista:
J Clin Apher
Año:
2024
Tipo del documento:
Article
País de afiliación:
Alemania