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Influence of support materials on the electroactive behavior, structure and gene expression of wild type and GSU1771-deficient mutant of Geobacter sulfurreducens biofilms.
Rodríguez-Torres, Luis Miguel; Huerta-Miranda, Guillermo Antonio; Martínez-García, Ana Luisa; Mazón-Montijo, Dalia Alejandra; Hernández-Eligio, Alberto; Miranda-Hernández, Margarita; Juárez, Katy.
Afiliación
  • Rodríguez-Torres LM; Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001. Col. Chamilpa, 62210, Cuernavaca, Morelos, México.
  • Huerta-Miranda GA; Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001. Col. Chamilpa, 62210, Cuernavaca, Morelos, México.
  • Martínez-García AL; Centro de Investigación en Materiales Avanzados S. C., Subsede Monterrey, Grupo de Investigación DORA-Lab, 66628, Apodaca, N. L, México.
  • Mazón-Montijo DA; Centro de Investigación e Innovación Tecnológica (CIIT), Grupo de Investigación DORA-Lab, Tecnológico Nacional de México Campus Nuevo León (TECNL), 66629, Apodaca, N. L, México.
  • Hernández-Eligio A; Centro de Investigación en Materiales Avanzados S. C., Subsede Monterrey, Grupo de Investigación DORA-Lab, 66628, Apodaca, N. L, México.
  • Miranda-Hernández M; Centro de Investigación e Innovación Tecnológica (CIIT), Grupo de Investigación DORA-Lab, Tecnológico Nacional de México Campus Nuevo León (TECNL), 66629, Apodaca, N. L, México.
  • Juárez K; Investigadores Por México, CONAHCYT, Ciudad de México, México.
Article en En | MEDLINE | ID: mdl-38758442
ABSTRACT
Geobacter sulfurreducens DL1 is a metal-reducing dissimilatory bacterium frequently used to produce electricity in bioelectrochemical systems (BES). The biofilm formed on electrodes is one of the most important factors for efficient electron transfer; this is possible due to the production of type IV pili and c-type cytochromes that allow it to carry out extracellular electron transfer (EET) to final acceptors. In this study, we analyzed the biofilm formed on different support materials (glass, hematite (Fe2O3) on glass, fluorine-doped tin oxide (FTO) semiconductor glass, Fe2O3 on FTO, graphite, and stainless steel) by G. sulfurreducens DL1 (WT) and GSU1771-deficient strain mutant (Δgsu1771). GSU1771 is a transcriptional regulator that controls the expression of several genes involved in electron transfer. Different approaches and experimental tests were carried out with the biofilms grown on the different support materials including structure analysis by confocal laser scanning microscopy (CLSM), characterization of electrochemical activity, and quantification of relative gene expression by RT-qPCR. The gene expression of selected genes involved in EET was analyzed, observing an overexpression of pgcA, omcS, omcM, and omcF from Δgsu1771 biofilms compared to those from WT, also the overexpression of the epsH gene, which is involved in exopolysaccharide synthesis. Although we observed that for the Δgsu1771 mutant strain, the associated redox processes are similar to the WT strain, and more current is produced, we think that this could be associated with a higher relative expression of certain genes involved in EET and in the production of exopolysaccharides despite the chemical environment where the biofilm develops. This study supports that G. sulfurreducens is capable of adapting to the electrochemical environment where it grows.
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Texto completo: 1 Bases de datos: MEDLINE Idioma: En Revista: Environ Sci Pollut Res Int Asunto de la revista: SAUDE AMBIENTAL / TOXICOLOGIA Año: 2024 Tipo del documento: Article

Texto completo: 1 Bases de datos: MEDLINE Idioma: En Revista: Environ Sci Pollut Res Int Asunto de la revista: SAUDE AMBIENTAL / TOXICOLOGIA Año: 2024 Tipo del documento: Article