Shp2 contributes to the regulation of nuclear shape and cellular viscoelasticity in response to substrate spatial cues.
Biochem Biophys Res Commun
; 721: 150144, 2024 Aug 20.
Article
en En
| MEDLINE
| ID: mdl-38781661
ABSTRACT
Cell polarization can be guided by substrate topology through space constraints and adhesion induction, which are part of cellular mechanosensing pathways. Here, we demonstrated that protein tyrosine phosphatase Shp2 plays a crucial role in mediating the response of cells to substrate spatial cues. When compared to cells spreading on surfaces coated uniformly with fibronectin (FN), cells attached to 10 µm-width FN-strip micropattern (MP), which provides spatial cues for uniaxial spreading, exhibited elongated focal adhesions (FAs) and aligned stress fibers in the direction of the MP. As a result of uniaxial cell spreading, nuclei became elongated, dependent on ROCK-mediated actomyosin contractility. Additionally, intracellular viscoelasticity also increased. Shp2-deficient cells did not display elongated FAs mediated by MP, well-aligned stress fibers, or changes in nuclear shape and intracellular viscoelasticity. Overall, our data suggest that Shp2 is involved in regulating FAs and the actin cytoskeleton to modulate nuclear shape and intracellular physical properties in response to substrate spatial cues.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Núcleo Celular
/
Adhesiones Focales
/
Elasticidad
/
Proteína Tirosina Fosfatasa no Receptora Tipo 11
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2024
Tipo del documento:
Article
País de afiliación:
Taiwán