Eml1 promotes axonal growth by enhancing αTAT1-mediated microtubule acetylation.
Biochim Biophys Acta Mol Cell Res
; 1871(7): 119770, 2024 Oct.
Article
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| MEDLINE
| ID: mdl-38897390
ABSTRACT
Microtubule stabilization is critical for axonal growth and regeneration, and many microtubule-associated proteins are involved in this process. In this study, we found that the knockdown of echinoderm microtubule-associated protein-like 1 (EML1) hindered axonal growth in cultured cortical and dorsal root ganglion neurons. We further revealed that EML1 facilitated the acetylation of microtubules and that the impairment of axonal growth due to EML1 inhibition could be restored by treatment with deacetylase inhibitors, suggesting that EML1 affected tubulin acetylation. Moreover, we verified an interaction between EML1 and the alpha-tubulin acetyltransferase 1, which is responsible for the acetylation of alpha-tubulin. We thus proposed that EML1 might regulate microtubule acetylation and stabilization via alpha-tubulin acetyltransferase 1 and then promote axon growth. Finally, we verified that the knockdown of EML1 in vivo also inhibited sciatic nerve regeneration. Our findings revealed a novel effect of EML1 on microtubule acetylation during axonal regeneration.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Acetiltransferasas
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Axones
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Sistema de Transporte de Aminoácidos A
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Proteínas Asociadas a Microtúbulos
Límite:
Animals
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Humans
Idioma:
En
Revista:
Biochim Biophys Acta Mol Cell Res
Año:
2024
Tipo del documento:
Article
País de afiliación:
China