Gut microbial metalloproteins and its role in xenobiotics degradation and ROS scavenging.
Adv Protein Chem Struct Biol
; 141: 495-538, 2024.
Article
en En
| MEDLINE
| ID: mdl-38960484
ABSTRACT
The gut microbial metalloenzymes play an important role in maintaining the balance between gut microbial ecosystem, human physiologically processes and immune system. The metals coordinated into active site contribute in various detoxification and defense strategies to avoid unfavourable environment and ensure bacterial survival in human gut. Metallo-ß-lactamase is a potent degrader of antibiotics present in periplasmic space of both commensals and pathogenic bacteria. The resistance to anti-microbial agents developed in this enzyme is one of the global threats for human health. The organophosphorus eliminator, organophosphorus hydrolases have evolved over a course of time to hydrolyze toxic organophosphorus compounds and decrease its effect on human health. Further, the redox stress responders namely superoxide dismutase and catalase are key metalloenzymes in reducing both endogenous and exogenous oxidative stress. They hold a great importance for pathogens as they contribute in pathogenesis in human gut along with reduction of oxidative stress. The in-silico study on these enzymes reveals the importance of point mutation for the evolution of these enzymes in order to enhance their enzyme activity and stability. Various mutation studies were conducted to investigate the catalytic activity of these enzymes. By using the "directed evolution" method, the enzymes involved in detoxification and defense system can be engineered to produce new variants with enhance catalytic features, which may be used to predict the severity due to multi-drug resistance and degradation pattern of organophosphorus compounds in human gut.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Xenobióticos
/
Especies Reactivas de Oxígeno
/
Microbioma Gastrointestinal
/
Metaloproteínas
Límite:
Humans
Idioma:
En
Revista:
Adv Protein Chem Struct Biol
/
Advances in protein chemistry and structural biology (Online)
Asunto de la revista:
BIOLOGIA
/
BIOQUIMICA
Año:
2024
Tipo del documento:
Article
País de afiliación:
India