Microsecond time-resolved X-ray scattering by utilizing MHz repetition rate at second-generation XFELs.
Nat Methods
; 2024 Jul 05.
Article
en En
| MEDLINE
| ID: mdl-38969722
ABSTRACT
Detecting microsecond structural perturbations in biomolecules has wide relevance in biology, chemistry and medicine. Here we show how MHz repetition rates at X-ray free-electron lasers can be used to produce microsecond time-series of protein scattering with exceptionally low noise levels of 0.001%. We demonstrate the approach by examining JÉ helix unfolding of a light-oxygen-voltage photosensory domain. This time-resolved acquisition strategy is easy to implement and widely applicable for direct observation of structural dynamics of many biochemical processes.
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MEDLINE
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En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2024
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Article
País de afiliación:
Suecia