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Microsecond time-resolved X-ray scattering by utilizing MHz repetition rate at second-generation XFELs.
Konold, Patrick E; Monrroy, Leonardo; Bellisario, Alfredo; Filipe, Diogo; Adams, Patrick; Alvarez, Roberto; Bean, Richard; Bielecki, Johan; Bódizs, Szabolcs; Ducrocq, Gabriel; Grubmueller, Helmut; Kirian, Richard A; Kloos, Marco; Koliyadu, Jayanath C P; Koua, Faisal H M; Larkiala, Taru; Letrun, Romain; Lindsten, Fredrik; Maihöfer, Michael; Martin, Andrew V; Mészáros, Petra; Mutisya, Jennifer; Nimmrich, Amke; Okamoto, Kenta; Round, Adam; Sato, Tokushi; Valerio, Joana; Westphal, Daniel; Wollter, August; Yenupuri, Tej Varma; You, Tong; Maia, Filipe; Westenhoff, Sebastian.
Afiliación
  • Konold PE; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden.
  • Monrroy L; Department of Chemistry - BMC, Uppsala University, Uppsala, Sweden.
  • Bellisario A; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden.
  • Filipe D; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden.
  • Adams P; School of Science, STEM College, RMIT University, Melbourne, Victoria, Australia.
  • Alvarez R; Department of Physics, Arizona State University, Tempe, AZ, USA.
  • Bean R; European XFEL, Schenefeld, Germany.
  • Bielecki J; European XFEL, Schenefeld, Germany.
  • Bódizs S; Department of Chemistry - BMC, Uppsala University, Uppsala, Sweden.
  • Ducrocq G; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
  • Grubmueller H; Department of Computer and Information Science (IDA), Linköping University, Linköping, Sweden.
  • Kirian RA; The Division of Statistics and Machine Learning (STIMA), Linköping University, Linköping, Sweden.
  • Kloos M; Department of Computer and Information Science (IDA), Linköping University, Linköping, Sweden.
  • Koliyadu JCP; European XFEL, Schenefeld, Germany.
  • Koua FHM; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
  • Larkiala T; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
  • Letrun R; European XFEL, Schenefeld, Germany.
  • Lindsten F; Department of Chemistry - BMC, Uppsala University, Uppsala, Sweden.
  • Maihöfer M; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
  • Martin AV; European XFEL, Schenefeld, Germany.
  • Mészáros P; Department of Computer and Information Science (IDA), Linköping University, Linköping, Sweden.
  • Mutisya J; The Division of Statistics and Machine Learning (STIMA), Linköping University, Linköping, Sweden.
  • Nimmrich A; Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.
  • Okamoto K; School of Science, STEM College, RMIT University, Melbourne, Victoria, Australia.
  • Round A; Department of Chemistry - BMC, Uppsala University, Uppsala, Sweden.
  • Sato T; Department of Chemistry - BMC, Uppsala University, Uppsala, Sweden.
  • Valerio J; Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
  • Westphal D; Department of Chemistry, University of Washington, Seattle, WA, USA.
  • Wollter A; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden.
  • Yenupuri TV; European XFEL, Schenefeld, Germany.
  • You T; European XFEL, Schenefeld, Germany.
  • Maia F; European XFEL, Schenefeld, Germany.
  • Westenhoff S; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden.
Nat Methods ; 2024 Jul 05.
Article en En | MEDLINE | ID: mdl-38969722
ABSTRACT
Detecting microsecond structural perturbations in biomolecules has wide relevance in biology, chemistry and medicine. Here we show how MHz repetition rates at X-ray free-electron lasers can be used to produce microsecond time-series of protein scattering with exceptionally low noise levels of 0.001%. We demonstrate the approach by examining Jɑ helix unfolding of a light-oxygen-voltage photosensory domain. This time-resolved acquisition strategy is easy to implement and widely applicable for direct observation of structural dynamics of many biochemical processes.
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Texto completo: 1 Bases de datos: MEDLINE Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2024 Tipo del documento: Article País de afiliación: Suecia
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Bases de datos: MEDLINE Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2024 Tipo del documento: Article País de afiliación: Suecia