Direct Somatic Embryogenesis in Carica papaya L. Genotypes for Genetic Modification Purposes.
Methods Mol Biol
; 2827: 279-290, 2024.
Article
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| MEDLINE
| ID: mdl-38985277
ABSTRACT
This chapter presents an efficient protocol for regenerating Carica papaya plants via somatic embryogenesis from immature zygotic embryos from economically important papaya genotypes. To achieve regenerated plants from somatic embryos, in the present protocol, four induction cycles are required, followed by one multiplication cycle and one regeneration cycle. With this optimized protocol, 80% of somatic embryos can be obtained in only 3.5 months. At this stage, calli containing more than 50% globular structures can be used for transformation (via agrobacterium, biobalistics, or any other transformation method). Once transformed, calli can be transferred to the following steps (multiplication, elongation, maturation, rooting, and ex vitro acclimatization) to regenerate a transformed somatic embryo-derived full plant.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Carica
/
Técnicas de Embriogénesis Somática de Plantas
/
Genotipo
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2024
Tipo del documento:
Article
País de afiliación:
México