RNA thermometers are widespread upstream of ABC transporter genes in bacteria.
J Biol Chem
; 300(8): 107547, 2024 Jul 09.
Article
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| MEDLINE
| ID: mdl-38992441
ABSTRACT
RNA thermometers are temperature-sensing non-coding RNAs that regulate the expression of downstream genes. A well-characterized RNA thermometer motif discovered in bacteria is the ROSE-like element (repression of heat shock gene expression). ATP-binding cassette (ABC) transporters are a superfamily of transmembrane proteins that harness ATP hydrolysis to facilitate the export and import of substrates across cellular membranes. Through structure-guided bioinformatics, we discovered that ROSE-like RNA thermometers are widespread upstream of ABC transporter genes in bacteria. X-ray crystallography, biochemistry, and cellular assays indicate that these RNA thermometers are functional regulatory elements. This study expands the known biological role of RNA thermometers to these key membrane transporters.
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MEDLINE
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Revista:
J Biol Chem
Año:
2024
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Article
País de afiliación:
Estados Unidos