Earthworm injury test for in-situ biomonitoring of pesticides in biobeds.
Chemosphere
; 363: 142923, 2024 Sep.
Article
en En
| MEDLINE
| ID: mdl-39059642
ABSTRACT
Biobeds are presented as an alternative for good pesticide wastewater management on farms. This work proposes a new test for in-situ biomonitoring of pesticide detoxification in biobeds. It is based on the assessment of visually appreciable injuries to Eisenia fetida. The severity of the injury to each exposed individual is assessed from the morphological changes observed in comparison with the patterns established in seven categories and, an injury index is calculated. A linear relationship between the proposed injury index and the pesticide concentration was determined for each pesticide sprayed individually in the biomixture. The five pesticides used were atrazine, prometryn, clethodim, haloxyfop-P-methyl and dicamba. In addition, a multiple linear regression model (i.e., a multivariate response surface) was fitted, which showed a good generalization capacity. The sensitivity range of the injury test was tested from 0.01 to 630 mg kg-1 as the total pesticide concentration. This index is then used to monitor the detoxification of these pesticides in a biomixture (composed of wheat stubble, river waste, and soil, 502525% by volume) over 210 days. The results are compared with standardized tests (Eisenia fetida avoidance test and Lactuca sativa seed germination test) carried out on the same biomixture. The results are also compared with data on the removal of pesticides. The injury test showed a better correlation with the removal of pesticides than the avoidance test and seed germination test. This simple and inexpensive test has proved to be useful for decontamination in-situ monitoring in biobeds.
Palabras clave
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Oligoquetos
/
Plaguicidas
/
Monitoreo Biológico
Límite:
Animals
Idioma:
En
Revista:
Chemosphere
Año:
2024
Tipo del documento:
Article
País de afiliación:
Argentina