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CGRP promotes osteogenic differentiation by regulating macrophage M2 polarization through HDAC6/AKAP12 signaling pathway.
Chen, Wenjing; Ma, Lin; Sun, Wencai; Xiao, Wenlong; Guo, Hao; Xiu, Jiang; Jiang, Xin.
Afiliación
  • Chen W; Department of Pathology, Qiqihar First Hospital, Qiqihar, 161005, Helongjiang Province, PR China.
  • Ma L; Department of Anesthesiology, Third Affiliated Hospital of Qiqihar Medical College, Qiqihar, 161000, Helongjiang Province, PR China.
  • Sun W; The Fifth department of Orthopaedics & Traumatology, Third Affiliated Hospital of Qiqihar Medical College, Qiqihar, 161000, Helongjiang Province, PR China.
  • Xiao W; The Fifth department of Orthopaedics & Traumatology, Third Affiliated Hospital of Qiqihar Medical College, Qiqihar, 161000, Helongjiang Province, PR China.
  • Guo H; The Fifth department of Orthopaedics & Traumatology, Third Affiliated Hospital of Qiqihar Medical College, Qiqihar, 161000, Helongjiang Province, PR China.
  • Xiu J; The Fifth department of Orthopaedics & Traumatology, Third Affiliated Hospital of Qiqihar Medical College, Qiqihar, 161000, Helongjiang Province, PR China.
  • Jiang X; The Fifth department of Orthopaedics & Traumatology, Third Affiliated Hospital of Qiqihar Medical College, Qiqihar, 161000, Helongjiang Province, PR China.
Regen Med ; 19(7-8): 379-391, 2024.
Article en En | MEDLINE | ID: mdl-39072399
ABSTRACT

Aim:

To determine the mechanism of Calcitonin gene-related peptide (CGRP) in bone healing.Materials &

methods:

Alkaline phosphatase (ALP) activity and inflammatory-factor levels were detected using ELISA. Osteogenic differentiation was assessed using Alizarin red staining technique. The interaction between histone deacetylase 6 (HDAC6) and A-kinase anchoring protein 12 (AKAP12) was investigated through Co- immunoprecipitation.

Results:

CGRP treatment promoted rat bone marrow-derived macrophages (BMDMs) M2 polarization. CGRP facilitated osteogenic differentiation by enhancing M2 polarization of BMDMs. Mechanistically, CGRP promoted AKAP12 acetylation to activate the extracellular regulated protein kinases pathway by HDAC6 inhibition.

Conclusion:

CGRP promoted M2 polarization of rat BMDMs and facilitated osteogenic differentiation through the HDAC6/AKAP12/extracellular regulated protein kinases signaling pathway, thereby promoting bone healing.
[Box see text].
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Osteogénesis / Transducción de Señal / Péptido Relacionado con Gen de Calcitonina / Diferenciación Celular / Proteínas de Anclaje a la Quinasa A / Macrófagos Límite: Animals Idioma: En Revista: Regen Med Año: 2024 Tipo del documento: Article
Texto completo
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Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Osteogénesis / Transducción de Señal / Péptido Relacionado con Gen de Calcitonina / Diferenciación Celular / Proteínas de Anclaje a la Quinasa A / Macrófagos Límite: Animals Idioma: En Revista: Regen Med Año: 2024 Tipo del documento: Article