Presynaptic Nrxn3 is essential for ribbon-synapse maturation in hair cells.
Development
; 151(19)2024 Oct 01.
Article
en En
| MEDLINE
| ID: mdl-39254120
ABSTRACT
Hair cells of the inner ear and lateral-line system rely on specialized ribbon synapses to transmit sensory information to the central nervous system. The molecules required to assemble these synapses are not fully understood. We show that Nrxn3, a presynaptic adhesion molecule, is crucial for ribbon-synapse maturation in hair cells. In both mouse and zebrafish models, the loss of Nrxn3 results in significantly fewer intact ribbon synapses. We show in zebrafish that, initially, Nrxn3 loss does not alter pre- and postsynapse numbers but, later, synapses fail to pair, leading to postsynapse loss. We also demonstrate that Nrxn3 subtly influences synapse selectivity in zebrafish lateral-line hair cells that detect anterior flow. Loss of Nrxn3 leads to a 60% loss of synapses in zebrafish, which dramatically reduces pre- and postsynaptic responses. Despite fewer synapses, auditory responses in zebrafish and mice are unaffected. This work demonstrates that Nrxn3 is a crucial and conserved molecule required for the maturation of ribbon synapses. Understanding how ribbon synapses mature is essential to generating new therapies to treat synaptopathies linked to auditory or vestibular dysfunction.
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Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Sinapsis
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Pez Cebra
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Proteínas de Pez Cebra
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Células Ciliadas Auditivas
Límite:
Animals
Idioma:
En
Revista:
Development
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Development (Camb.)
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Development (Cambridge)
Asunto de la revista:
BIOLOGIA
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EMBRIOLOGIA
Año:
2024
Tipo del documento:
Article
País de afiliación:
Estados Unidos