Purification of the structural and nonstructural proteins of St. Louis encephalitis virus.
J Virol Methods
; 9(4): 283-91, 1984 Dec.
Article
en En
| MEDLINE
| ID: mdl-6099371
ABSTRACT
We have developed a procedure for purifying both the structural and nonstructural proteins of flaviviruses from lysates of infected cell cultures. The procedure involves immunoprecipitation to concentrate viral proteins and eliminate most of the cellular proteins, preparative polyacrylamide gel electrophoresis to separate the viral proteins, and hydroxyapatite chromatography, which eliminates most of the unlabeled cellular protein. This procedure offers an improvement over previous purification schemes in that there is no loss of viral proteins after the immunoprecipitation step, any combination of labeling isotopes may be used, and it is not necessary to soak proteins out of gel slices.
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Bases de datos:
MEDLINE
Asunto principal:
Proteínas Virales
/
Virus de la Encefalitis de San Luis
/
Flavivirus
Idioma:
En
Revista:
J Virol Methods
Año:
1984
Tipo del documento:
Article