Prolyl and lysyl hydroxylase activities of human skin fibroblasts: effect of donor age and ascorbate.
J Invest Dermatol
; 75(5): 404-7, 1980 Nov.
Article
en En
| MEDLINE
| ID: mdl-6253574
ABSTRACT
Prolyl and lysyl hydroxylase activities in cultures of human skin fibroblasts from fetal to 94-yr-old donors were measured. In contrast to earlier studies with whole skin, neither prolyl nor lysyl hydroxylase activity was found related to donor age. Prolyl hydroxylase activity increased 3- to 6-fold when cell extracts were incubated with ascorbate and other hydroxylation cofactors before assay. A similar increase in prolyl hydroxylase activity occurred when cells were incubated with ascorbate. Lysyl hydroxylase activity remained unaltered under these conditions.
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Bases de datos:
MEDLINE
Asunto principal:
Ácido Ascórbico
/
Piel
/
Procolágeno-Lisina 2-Oxoglutarato 5-Dioxigenasa
/
Procolágeno-Prolina Dioxigenasa
/
Oxigenasas de Función Mixta
Límite:
Adolescent
/
Adult
/
Aged
/
Child
/
Child, preschool
/
Humans
/
Infant
/
Middle aged
/
Newborn
Idioma:
En
Revista:
J Invest Dermatol
Año:
1980
Tipo del documento:
Article