Purification and characterization of an endothelial cell-viability maintaining factor from fetal bovine serum.
Biochim Biophys Acta
; 1269(1): 13-8, 1995 Oct 19.
Article
en En
| MEDLINE
| ID: mdl-7578265
ABSTRACT
Serum is an essential requirement for the growth and long-term survival of human endothelial cells, even in the presence of such defined elements such as polypeptide growth factors and hormones. A polypeptide from fetal bovine serum was isolated and characterized on the basis of long-term survival of human endothelial cells in serum-free culture. The endothelial cell viability maintaining factor has been purified to homogeneity by a combination of polyethylene glycol precipitation, hydroxylapatite, gel permeation and reverse-phase high performance liquid chromatography. The final purified endothelial cell viability maintaining factor has a molecular weight of 65,000 (reduced) and has been identified as bovine apolipoprotein H by amino-terminal amino acid sequence analysis and Western blot analysis. Endothelial cell viability maintaining factor improved a long-term viability of human endothelial cells at maximal concentrations of 2.5-5 micrograms protein/ml in serum-free medium.
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Bases de datos:
MEDLINE
Asunto principal:
Glicoproteínas
/
Supervivencia Celular
/
Sangre Fetal
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1995
Tipo del documento:
Article
País de afiliación:
Japón