Secretion of bioactive granulocyte-macrophage colony-stimulating factor by human colorectal carcinoma cells.
Cancer Res
; 54(14): 3700-2, 1994 Jul 15.
Article
en En
| MEDLINE
| ID: mdl-8033086
ABSTRACT
Secretion of several cytokines by colorectal carcinoma cells has been substantiated. These do not include granulocyte-macrophage colony-stimulating factor (GM-CSF) thus far. We show that the supernatant of two human colorectal carcinoma cell lines, LS1034 and SW480, stimulates proliferation of GM-CSF-dependent M07e cells. The activity was constitutively secreted by LS1034 cells and could be induced by serum-free culture conditions in SW480 cells. Addition of a neutralizing anti-GM-CSF antibody completely inhibited this activity. Preabsorption with anti-GM-CSF antibody removed all M07e growth-stimulating activity from LS1034 and SW480 supernatant. Western blot analysis revealed the presence of GM-CSF in LS1034 supernatant. Our results indicate that human colorectal carcinoma cells secrete indeed biologically active GM-CSF.
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Bases de datos:
MEDLINE
Asunto principal:
Neoplasias Colorrectales
/
Factor Estimulante de Colonias de Granulocitos y Macrófagos
Límite:
Humans
Idioma:
En
Revista:
Cancer Res
Año:
1994
Tipo del documento:
Article