Improved methods for the cultivation of strictly anaerobic, extremely thermophilic methanogens.
Biotechniques
; 15(6): 996-8, 1000, 1002, 1993 Dec.
Article
en En
| MEDLINE
| ID: mdl-8292353
The strictly anaerobic, extremely thermophilic methanogens, Methanobacterium thermoautotrophicum Marburg and M. thermoautotrophicum delta H, have been cultivated in liquid culture and on solid medium in screw-top bottles, which permit continuous monitoring of the growth of the microorganisms. We have been able to routinely grow methanogens in medium containing bicarbonate, TRIS or 4-morpholinepropanesulfonic acid (MOPS) buffers and three different sulfur sources (sulfide, sulfite and thiosulfate) at temperatures up to 70 degrees C and at pressures up to 35 psi while monitoring cell density or colony formation.
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Bases de datos:
MEDLINE
Asunto principal:
Técnicas Bacteriológicas
/
Methanobacterium
Idioma:
En
Revista:
Biotechniques
Año:
1993
Tipo del documento:
Article