Oxidative stress evaluation of ischemia and reperfusion in kidneys under various degrees of hypothermia in rats
Acta cir. bras
; 28(8): 568-573, Aug. 2013. ilus, graf
Article
em En
| LILACS
| ID: lil-680610
Biblioteca responsável:
BR1.1
ABSTRACT
PURPOSE:
To design an animal model of ischemia-reperfusion (I/R) in kidneys and evaluate the role that predetermined ranges of local hypothermia plays on markers of stress-oxydative as well as on histologic sections.METHODS:
Twenty eight male rats Wistar, under general anesthesia, undergone right nephrectomy (G0, control group) followed by left kidney ischemia during 40 min. Four temperatures groups were designed, with seven animals randomized for each group normothermic (G1, ±37ºC), mild hypothermia (G2, 26ºC), moderate hypothermia (G3, 15ºC) and deep hypothermia (G4, 4ºC). Left kidney temperature was assessed with an intraparenchymal probe. Left nephrectomy was performed after 240 min of reperfusion. After I/R a blood sample was obtained for f2-IP. Half of each kidney was sent to pathological evaluation and half to analyze CAT, SOD, TBARS, NO3, NO2.RESULTS:
Histopathology showed that all kidneys under I/R were significantly more injured than the G0 (p<0.001). TBARS had increased levels in all I/R groups compared with the G0 (p<0.001). CAT had a significant difference (p<0.03) between G1 and G4. Finally, no difference was found on SOD, NO3, NO2 nor on f2-IP.CONCLUSION:
This model of I/R was efficient to produce oxidative-stress in the kidney, showing that 4ºC offered significant decrease in free radicals production, although tissue protection was not observed.Palavras-chave
Texto completo:
1
Bases de dados:
LILACS
Assunto principal:
Traumatismo por Reperfusão
/
Estresse Oxidativo
/
Hipotermia Induzida
/
Isquemia
/
Rim
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Acta cir. bras
Assunto da revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Brasil