A universal influenza A vaccine based on the extracellular domain of the M2 protein.
Nat Med
; 5(10): 1157-63, 1999 Oct.
Article
em En
| MEDLINE
| ID: mdl-10502819
The antigenic variation of influenza virus represents a major health problem. However, the extracellular domain of the minor, virus-coded M2 protein is nearly invariant in all influenza A strains. We genetically fused this M2 domain to the hepatitis B virus core (HBc) protein to create fusion gene coding for M2HBc; this gene was efficiently expressed in Escherichia coli. Intraperitoneal or intranasal administration of purified M2HBc particles to mice provided 90-100% protection against a lethal virus challenge. The protection was mediated by antibodies, as it was transferable by serum. The enhanced immunogenicity of the M2 extracellular domain exposed on HBc particles allows broad-spectrum, long-lasting protection against influenza A infections.
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Bases de dados:
MEDLINE
Assunto principal:
Vírus da Influenza A
/
Vacinas contra Influenza
/
Proteínas da Matriz Viral
/
Infecções por Orthomyxoviridae
Limite:
Animals
Idioma:
En
Revista:
Nat Med
Assunto da revista:
BIOLOGIA MOLECULAR
/
MEDICINA
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Bélgica