High-performance liquid chromatography with amperometric detection applied to the screening of 1,4-dihydropyridines in human plasma.
J Chromatogr A
; 870(1-2): 105-14, 2000 Feb 18.
Article
em En
| MEDLINE
| ID: mdl-10722067
ABSTRACT
A high-performance liquid chromatographic method with electrochemical detection has been developed for the determination of six 1,4-dihydropyridines nifedipine, nimodipine, nisoldipine, nicardipine, felodipine and lacidipine. The chromatographic separation was performed using a Supelcosil LC-ABZ+Plus C18 column. A mobile phase of methanol-water (7030), containing 2 mM CH3COOH-CH3COONa at a flow-rate of 1 ml/min and a pH of 5.0, was used. The temperature was optimized at 30+/-0.2 degrees C. The amperometric detector, equipped with a glassy carbon electrode, was operated at 1000 mV versus Ag/AgCl in the direct current mode. The method was applied to the determination of these compounds at ng/ml concentrations, obtaining intra-day reproducibilities of lower than 5.0% in terms of relative standard deviations and detection limits ranging from 16 to 44 ng/ml. The method was applied to the screening of 1,4-dihydropyridines in spiked plasma samples, with a total elution time of lower than 18 min, obtaining the best recoveries for nimodipine and felodipine (91 and 88%, respectively). These recoveries together with the low detection limits achieved allow its application to the analysis of these drugs in human plasma.
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Bases de dados:
MEDLINE
Assunto principal:
Di-Hidropiridinas
/
Bloqueadores dos Canais de Cálcio
/
Cromatografia Líquida de Alta Pressão
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Humans
Idioma:
En
Revista:
J Chromatogr A
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Espanha