Evidence of a tissue-restricting DNA regulatory element in the mouse IRBP promoter.
FEBS Lett
; 504(1-2): 27-30, 2001 Aug 24.
Article
em En
| MEDLINE
| ID: mdl-11522290
ABSTRACT
The expression of interphotoreceptor retinoid binding protein (IRBP) is limited to photoreceptor cells of the retina and pinealocytes of the pineal gland. We sought to define cis-elements of the mouse IRBP 5' flanking region that are required for this restricted activity. In vitro transient transfections of retinoblastoma and neuroblastoma cells and in vivo experiments with transgenic Xenopus laevis indicate that -1783/+101 and -156/+101 IRBP gene fragments directed expression predominantly to the retina and pineal, with minor neuronal expression elsewhere. In contrast, a -70/+101 fragment was less restrictive in controlling expression, exhibiting activity not only in retina, but also in forebrain, hindbrain, spinal cord, and motor neurons innervating gills.
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Bases de dados:
MEDLINE
Assunto principal:
Proteínas de Ligação ao Retinol
/
Regiões Promotoras Genéticas
/
Proteínas do Olho
Limite:
Animals
/
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Estados Unidos