Antibody fusions with fluorescent proteins: a versatile reagent for profiling protein expression.
J Immunol Methods
; 257(1-2): 175-84, 2001 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-11687251
ABSTRACT
We developed a system by which antibodies, fused to fluorescent proteins with different wavelengths, can be prepared within a month against various antigens. An antibody library composed of a large number of single-chain Fv-CL fragment was constructed by means of a phage-display system. The constructs were designed to facilitate changing of the protein forms by simple enzyme manipulation. In the present study, we adopted a molecular form of antibody in which a single-chain Fv-CL fragment is fused with a green fluorescent protein (GFP) or red fluorescent protein (RFP). In addition, a His-tag was inserted between CL and GFP (or RFP). We describe the utility of this system using Caenorhabditis elegans embryo as a model.
Buscar no Google
Bases de dados:
MEDLINE
Assunto principal:
Perfilação da Expressão Gênica
/
Proteínas Luminescentes
/
Anticorpos
Limite:
Animals
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Japão