Mai1p is essential for maturation of proaminopeptidase I but not for autophagy.
FEBS Lett
; 512(1-3): 173-9, 2002 Feb 13.
Article
em En
| MEDLINE
| ID: mdl-11852075
ABSTRACT
We here identify Mai1p, a homologue of the autophagy protein Aut10p, as a novel component essential for proaminopeptidase I (proAPI) maturation under non-starvation conditions. In mai1Delta cells mature vacuolar proteinases are detectable and vacuolar acidification is normal. In mai1Delta cells autophagy occurs, though at a somewhat reduced level. This is indicated by proAPI maturation during starvation and accumulation of autophagic bodies during starvation with phenylmethylsulfonyl fluoride. Homozygous diploid mai1Delta cells sporulate, but with a slightly reduced frequency. Biologically active Ha-tagged Mai1p, chromosomally expressed under its native promoter, is at least in part peripherally membrane-associated. In indirect immunofluorescence it localizes to the vacuolar membrane or structures nearby. In some cells Ha-tagged Mai1p appears concentrated at regions adjacent to the nucleus.
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Bases de dados:
MEDLINE
Assunto principal:
Endopeptidases
/
Precursores de Proteínas
/
Autofagia
/
Proteínas de Saccharomyces cerevisiae
/
Aminopeptidases
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Alemanha