Application of 1D 1H NMR for fast non-targeted screening and compositional analysis of steroid cocktails and veterinary drug formulations administered to livestock.

ABSTRACT

A fast non-targeted strategy is described for analysis of formulations--meant for administration to live stock--containing growth-promoting agents or veterinary drugs. The use of 1H NMR as a first step universal screening method is applied and used in routine analysis. The implementation of this approach has increased the analysis efficiency considerably. Apart from screening on illegal compounds, 1H NMR information on matrix and thus, indirectly, administration mode, can be present. An ever-growing 1H NMR database is used containing more than 200 reference substances. Based on the 1H NMR screening, decisions for further analysis can be made, such as for instance HPLC fractionation of steroid cocktails and subsequent 1H NMR (and LC-MS) analysis. Examples of unravelling formulations are given in detail including a steroid cocktail containing 15 compounds.