Rapid screening and identification of cytochalasins by electrospray tandem mass spectrometry.
J Mass Spectrom
; 37(3): 283-91, 2002 Mar.
Article
em En
| MEDLINE
| ID: mdl-11921369
ABSTRACT
The cytochalasin class of fungal metabolites was analyzed by electrospray ionization tandem mass spectrometry (ESI-MS/MS) with the aim of developing a methodology for their rapid identification in microbial extracts. ESI-MS analyses of reference cytochalasins were performed and several product ions were produced in MS/MS experiments on parent ions that are structurally characteristic. A precursor ion search was performed to detect cytochalasins in an ethyl acetate extract of fungal strain RK97-F21. Three cytochalasins were detected and one of the components was identified as epoxycytochalasin H by comparing the tandem mass spectra of the product ions with those of reference compounds. This finding was further validated by LC/MS and LC/MS/MS experiments.
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Bases de dados:
MEDLINE
Assunto principal:
Citocalasinas
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Idioma:
En
Revista:
J Mass Spectrom
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Japão