Application of over-run thin-layer and gas-liquid chromatography in the separation of closely related C19O2 steroids.
J Chromatogr
; 115(1): 177-82, 1975 Dec 10.
Article
em En
| MEDLINE
| ID: mdl-1202060
ABSTRACT
An improved method for the separation of epimeric C19O2 steroids and their related allylic alcohols is described. In this method, the steroids are first separated by over-run thin-layer chromatography, and the unresolved groups are further analysed as free or as trimethylsilyl ether derivatives by gas-liquid chromatography. The behaviour of twenty-one C19O2 steroids was investigated by thin-layer chromatography in four systems and by gas-liquid chromatography in four liquid phases. All steroid pairs of similar polarity were resolved by the combination of these two fractionation procedures.
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Bases de dados:
MEDLINE
Assunto principal:
Androstanos
Idioma:
En
Revista:
J Chromatogr
Ano de publicação:
1975
Tipo de documento:
Article