Successful inactivation of endogenous Oct-3/4 and c-mos genes in mouse preimplantation embryos and oocytes using short interfering RNAs.
Biochem Biophys Res Commun
; 296(5): 1372-7, 2002 Sep 06.
Article
em En
| MEDLINE
| ID: mdl-12207927
ABSTRACT
Understanding oocyte maturation and early development in mammals is very important, especially because these cells serve as a source of materials useful in medical applications, such as ES cells. However, the limited availability of oocytes and embryos hampers the molecular dissection of the very early stage of mammalian development. Recently, the RNA interference technology has been acknowledged to be very effective and useful in diverse groups of cells, including mammalian cells. In this study, we examined whether short interfering RNAs (siRNAs) are applicable to mouse oocytes and preimplantation embryos, by targeting two genes, namely, Oct-3/4 and c-mos. siRNA injections successfully extinguished the production of these target genes. Moreover, the siRNA-injected oocytes and embryos showed phenotypes very similar to those exhibited by Oct-3/4- or Mos-knockout mice in previous studies. Accordingly, we concluded that siRNA is a useful tool in molecular studies on the early development of mouse.
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Bases de dados:
MEDLINE
Assunto principal:
Oócitos
/
Fatores de Transcrição
/
Blastocisto
/
Genes mos
/
RNA não Traduzido
Limite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Japão