Real-time measurement of spontaneous antigen-antibody dissociation.
Biophys J
; 83(4): 1965-73, 2002 Oct.
Article
em En
| MEDLINE
| ID: mdl-12324415
ABSTRACT
We report observations in real time of thermally driven adhesion and dissociation between a monoclonal IgE antibody and its specific antigen N-epsilon-2,4-dinitrophenyl-L-lysine. Both molecules were attached to the surfaces of different polystyrene microspheres trapped by optical tweezers. Monitoring spontaneous successive attachment and detachment events allowed a direct determination of the reaction-limited detachment rate k(off) for a single bond and also for multiple bonds. We observed both positive and negative cooperativity between multiple bonds depending on whether the antigen was linked to the microsphere with or without a tether, respectively.
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Bases de dados:
MEDLINE
Assunto principal:
Imunoglobulina E
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Lisina
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Reações Antígeno-Anticorpo
Idioma:
En
Revista:
Biophys J
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Estados Unidos