Imaging cell death with radiolabeled annexin V in an experimental model of rheumatoid arthritis.
J Nucl Med
; 43(10): 1359-65, 2002 Oct.
Article
em En
| MEDLINE
| ID: mdl-12368374
ABSTRACT
UNLABELLED Rheumatoid arthritis is associated with chronic synovial inflammation due to the abnormal accumulation of macrophages and autoreactive T lymphocytes in joints. The autoreactive cells cause an inflammatory proapoptotic response to self-antigens resulting in eventual bone, cartilage, and soft-tissue loss and destruction. The goal of our study was to determine the timing and intensity of apoptosis in joints using 99mTc-labeled annexin V, an in vivo marker of apoptosis, in a murine model of immune arthritis. METHODS:
We used 99mTc-annexin V and autoradiography to study the extent and severity of apoptosis in the front and rear paws of DBA/1 mice with type II collagen-induced rheumatoid arthritis.RESULTS:
Compared with control values (n = 10), there was a significant (P < 0.002) nearly 3-fold increase in uptake of 99mTc-annexin V in the front foot pads, rear toes, rear foot pads, and heels at the time of maximal extremity swelling as determined by serial caliper measurements at 4 wk after inoculation with type II bovine collagen (n = 9). The front toes had a 5- to 6-fold increase in uptake compared with control values (P < 0.001). Histologic analysis revealed only scattered rare lymphocytes in the periarticular soft tissues, without joint destruction. Dual autoradiography with 125I-bovine serum albumin as a control showed that 99mTc-annexin V localization was specific. Treatment with methylprednisolone for 1 wk (n = 8) at 4 wk after immunization with type II collagen decreased 99mTc-annexin V uptake by 3- to 6-fold compared with control values (P < 0.002).CONCLUSION:
99mTc-annexin V can detect collagen-induced immune arthritis and its response to steroid therapy before joint destruction.
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Bases de dados:
MEDLINE
Assunto principal:
Artrite Experimental
/
Artrite Reumatoide
/
Apoptose
/
Anexina A5
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
J Nucl Med
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Estados Unidos