Isolation and characterization of four cell wall purple acid phosphatase genes from tobacco cells.
Biochim Biophys Acta
; 1625(2): 134-40, 2003 Jan 27.
Article
em En
| MEDLINE
| ID: mdl-12531472
ABSTRACT
Four full-length cDNAs were isolated from a cDNA library prepared from tobacco cultured cells and designated NtPAP4, NtPAP12, NtPAP19 and NtPAP21, which could correspond to purple acid phosphatase (PAP). Levels of both NtPAP12 and NtPAP21 mRNA in the protoplasts immediately increased after the protoplasts were transferred to a medium for cell wall regeneration, and the accumulation of the mRNA was correlated with cell wall regeneration for 3 h. It is likely that the NtPAP12 and NtPAP21 gene products are wall-bound PAPs at the early stage of regenerating walls in tobacco protoplasts.
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Bases de dados:
MEDLINE
Assunto principal:
Nicotiana
/
Fosfatase Ácida
/
Glicoproteínas
/
Parede Celular
/
Genes de Plantas
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Japão