BATF transgenic mice reveal a role for activator protein-1 in NKT cell development.
J Immunol
; 170(5): 2417-26, 2003 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-12594265
ABSTRACT
The importance of regulated AP-1 activity during T cell development was assessed using transgenic mice overexpressing BATF, a basic leucine zipper transcription factor and an AP-1 inhibitor. BATF transgenic animals possess normal thymic cellularity and all major T cell subsets, but show impaired thymocyte proliferation in vitro and no induction of IL-2, IL-4, IL-5, IL-10, and IL-13 expression. Since NKT cells are largely responsible for cytokine production in the thymus, this population was examined by detection of the V alpha 14-J alpha 281 TCR, flow cytometry of NK1.1(+) TCR beta(+) cells, and analysis of cytokine production by heat-stable Ag(low) thymocytes and peripheral NKT cells stimulated in vivo. Results show a severe under-representation of NKT cells in BATF transgenic animals, providing the first evidence that the precise control of AP-1-mediated transcription is critical for the proper emergence of thymus-derived NKT cells in the mouse.
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Bases de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Camundongos Transgênicos
/
Células Matadoras Naturais
/
Subpopulações de Linfócitos T
/
Fator de Transcrição AP-1
Idioma:
En
Revista:
J Immunol
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Estados Unidos