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Ethanol modulates epidermal growth factor-stimulated tyrosine kinase and phosphorylation of PLC-gamma 1.
Thurston, A W; Shukla, S D.
Afiliação
  • Thurston AW; Department of Pharmacology, School of Medicine, University of Missouri, Columbia 65212.
Biochem Biophys Res Commun ; 185(3): 1062-8, 1992 Jun 30.
Article em En | MEDLINE | ID: mdl-1320873
A431 cells have an abundance of Epidermal Growth Factor (EGF) receptors which possess intrinsic tyrosine kinase activity. Treatment of membranes isolated from A431 cells with EGF caused a 2-3 fold increase in phosphorylation of a synthetic peptide (Arg-Arg-Leu-Ile-Glu-Asp-Ala-Glu-Tyr-Ala-Ala-Arg-Gly) which is a substrate for tyrosine kinase. Treatment of these membranes with 0.1 to 100 mM ethanol altered basal tyrosine kinase activity in a biphasic manner; increase at 10 mM and decrease at 100 mM ethanol. The treatment of the membranes with the same concentrations of ethanol also altered EGF's ability to stimulate tyrosine kinase activity: increase at 0.1 mM ethanol and decrease at 10 mM. Strikingly, EGF-stimulated tyrosine kinase was more sensitive to ethanol than the basal activity. Experiments with other alcohols showed a relationship between chain length and the inhibitory ability of the alcohol. These data demonstrate a biochemical effect of low concentrations of ethanol on tyrosine kinase. Interestingly, ethanol treatment of A431 cells inhibited EGF-stimulated phosphorylation of PLC-gamma 1 which is a substrate for EGF receptor tyrosine kinase. It is concluded that ethanol at low concentrations has significant modulatory effect on basal and EGF-stimulated tyrosine kinase, as well as PLC-gamma 1 phosphorylation.
Assuntos
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Bases de dados: MEDLINE Assunto principal: Fosfolipases Tipo C / Proteínas Tirosina Quinases / Etanol / Fator de Crescimento Epidérmico / Isoenzimas Limite: Humans Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 1992 Tipo de documento: Article
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Bases de dados: MEDLINE Assunto principal: Fosfolipases Tipo C / Proteínas Tirosina Quinases / Etanol / Fator de Crescimento Epidérmico / Isoenzimas Limite: Humans Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 1992 Tipo de documento: Article