Entry of lomefloxacin and temafloxacin into human neutrophils, peritoneal macrophages, and tissue culture cells.
Diagn Microbiol Infect Dis
; 15(5): 393-8, 1992 Jul.
Article
em En
| MEDLINE
| ID: mdl-1322808
ABSTRACT
The uptake of lomefloxacin (difluoroquinolone) and temafloxacin (trifluoroquinolone) by human polymorphonuclear leukocytes (PMNs), peritoneal macrophages (PM phis), and two tissue culture cells (McCoy and Vero) was measured by a fluorometric assay. Both antimicrobials reached high intracellular concentrations in PMNs [cellular to extracellular ratio (C/E) greater than 4], in PM phis (C/E greater than 3) and lower in tissue culture cells (C/E greater than 1) at an extracellular concentration of 5 mg/L. Lomefloxacin uptake by PMNs was more rapid than that of temafloxacin. Entry of both quinolones into PMNs was environmental temperature-dependent, but not affected by cell viability. Ingestion of opsonized Staphylococcus aureus did not affect the ability of PMNs to concentrate these antimicrobials. Ingestion of opsonized zymosan or stimulation with phorbol myristate acetate significantly increased the PMN association of both quinolones, this effect being particularly marked with temafloxacin. It is concluded that both lomefloxacin and temafloxacin are markedly concentrated within human phagocytes and tissue culture cells, although this phenomenon is not dependent on the degree of fluorination of the molecule.
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Bases de dados:
MEDLINE
Assunto principal:
Quinolonas
/
Fluoroquinolonas
/
Macrófagos
/
Anti-Infecciosos
/
Neutrófilos
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Diagn Microbiol Infect Dis
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Espanha