Continuous D-tagatose production by immobilized thermostable L-arabinose isomerase in a packed-bed bioreactor.
Biotechnol Prog
; 19(6): 1643-7, 2003.
Article
em En
| MEDLINE
| ID: mdl-14656135
ABSTRACT
D-Tagatose was continuously produced using thermostable L-arabinose isomerase immobilized in alginate with D-galactose solution in a packed-bed bioreactor. Bead size, L/D (length/diameter) of reactor, dilution rate, total loaded enzyme amount, and substrate concentration were found to be optimal at 0.8 mm, 520/7 mm, 0.375 h(-1), 5.65 units, and 300 g/L, respectively. Under these conditions, the bioreactor produced about 145 g/L tagatose with an average productivity of 54 g tagatose/L x h and an average conversion yield of 48% (w/w). Operational stability of the immobilized enzyme was demonstrated, with a tagatose production half-life of 24 days.
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Bases de dados:
MEDLINE
Assunto principal:
Cromatografia
/
Reatores Biológicos
/
Aldose-Cetose Isomerases
/
Geobacter
/
Galactose
/
Hexoses
Tipo de estudo:
Evaluation_studies
Idioma:
En
Revista:
Biotechnol Prog
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2003
Tipo de documento:
Article