Calculation of the molar absorptivity of polyphenols by using liquid chromatography with diode array detection: the case of carnosic acid.
J Chromatogr A
; 1023(2): 225-9, 2004 Jan 16.
Article
em En
| MEDLINE
| ID: mdl-14753688
ABSTRACT
Antioxidant activity of vegetable extracts is related to the nature and the amount of active components, mainly polyphenols; therefore, a correct quantification of these molecules should be required to define their concentration in such kind of vegetable extracts. A fast and accurate method to calculate molar absorption coefficients (epsilon), by using HPLC, has been tested on standard polyphenols and caffeine, and should be widely adapted for standardless quantitative analysis. Molar absorptivity (epsilon) of carnosic acid (CA) was determined from 200 to 300 nm, by the proposed method and those values were compared to tert-butyl-hydroxytoluene (BHT) ones for further comparative quantification.
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Bases de dados:
MEDLINE
Assunto principal:
Fenóis
/
Espectrofotometria Ultravioleta
/
Flavonoides
/
Cromatografia Líquida de Alta Pressão
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
J Chromatogr A
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Itália