Real-time PCR detection of ruminant DNA.
J Food Prot
; 67(3): 550-4, 2004 Mar.
Article
em En
| MEDLINE
| ID: mdl-15035372
ABSTRACT
To control the spread of bovine spongiform encephalopathy, several DNA methods have been described for the detection of the species origin of meat and bone meal. Most of these methods are based on the amplification of a mitochondrial DNA segment. We have developed a semiquantitative method based on real-time PCR for detection of ruminant DNA, targeting an 88-bp segment of the ruminant short interspersed nuclear element Bov-A2. This method is specific for ruminants and is able to detect as little as 10 fg of bovine DNA. Autoclaving decreased the amount of detectable DNA, but positive signals were observed in feeding stuff containing 10% bovine material if this had not been rendered in accordance with the regulations, i.e., heated at 134 degrees C for 3 instead of 20 min.
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Bases de dados:
MEDLINE
Assunto principal:
DNA Mitocondrial
/
Reação em Cadeia da Polimerase
/
Encefalopatia Espongiforme Bovina
/
Elementos Nucleotídeos Curtos e Dispersos
/
Ração Animal
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Food Prot
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Espanha