Characterization of the two distinct subtypes of metabotropic glutamate receptors from honeybee, Apis mellifera.
Neurosci Lett
; 359(3): 190-4, 2004 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-15050695
ABSTRACT
L-Glutamate is a major neurotransmitter at the excitatory synapses in the vertebrate brain. It is also the excitatory neurotransmitter at neuromuscular junctions in insects, however its functions in their brains remain to be established. We identified and characterized two different subtypes (AmGluRA and AmGluRB) of metabotropic glutamate receptors (mGluRs) from an eusocial insect, honeybee. Both AmGluRA and AmGluRB form homodimers independently on disulfide bonds, and bind [3H]glutamate with K(D) values of 156.7 and 80.7 nM, respectively. AmGluRB is specifically expressed in the brain, while AmGluRA is expressed in the brain and other body parts, suggesting that AmGluRA is also present at the neuromuscular junctions. Both mGluRs are expressed in the mushroom bodies and the brain regions of honeybees, where motor neurons are clustered. Their expression in the brain apparently overlaps, suggesting that they may interact with each other to modulate the glutamatergic neurotransmission.
Buscar no Google
Bases de dados:
MEDLINE
Assunto principal:
Filogenia
/
Abelhas
/
Encéfalo
/
Receptores de Glutamato Metabotrópico
/
Perfilação da Expressão Gênica
Limite:
Animals
Idioma:
En
Revista:
Neurosci Lett
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Japão